A long distance RT-PCR able to amplify the Pestivirus genome
نویسندگان
چکیده
منابع مشابه
Use of long RT-PCR to characterize splice variant mRNAs.
Recent advances in long reverse transcription (RT)-PCR technology allow the copying of full-length coding regions of large mRNAs in one step. Using long RT-PCR, one can be certain that a given cDNA is derived from a single mRNA. In what to our knowledge is a novel application, we can isolate and characterize splice variants for any given mRNA in a systematic manner. We optimized long RT-PCR to ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2006
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2006.01.005